Kinetics of substrate recognition and cleavage by human 8-oxoguanine-DNA glycosylase
Identifieur interne : 001768 ( Main/Exploration ); précédent : 001767; suivant : 001769Kinetics of substrate recognition and cleavage by human 8-oxoguanine-DNA glycosylase
Auteurs : Nikita A. Kuznetsov [Russie] ; Vladimir V. Koval [Russie] ; Dmitry O. Zharkov [Russie] ; Georgy A. Nevinsky [Russie] ; Kenneth T. Douglas [Royaume-Uni] ; Olga S. Fedorova [Russie]Source :
- Nucleic Acids Research [ 0305-1048 ] ; 2005.
Abstract
Human 8-oxoguanine-DNA glycosylase (hOgg1) excises 8-oxo-7,8-dihydroguanine (8-oxoG) from damaged DNA. We report a pre-steady-state kinetic analysis of hOgg1 mechanism using stopped-flow and enzyme fluorescence monitoring. The kinetic scheme for hOgg1 processing an 8-oxoG:C-containing substrate was found to include at least three fast equilibrium steps followed by two slow, irreversible steps and another equilibrium step. The second irreversible step was rate-limiting overall. By comparing data from Ogg1 intrinsic fluorescence traces and from accumulation of products of different types, the irreversible steps were attributed to two main chemical steps of the Ogg1-catalyzed reaction: cleavage of the N-glycosidic bond of the damaged nucleotide and β-elimination of its 3′-phosphate. The fast equilibrium steps were attributed to enzyme conformational changes during the recognition of 8-oxoG, and the final equilibrium, to binding of the reaction product by the enzyme. hOgg1 interacted with a substrate containing an aldehydic AP site very slowly, but the addition of 8-bromoguanine (8-BrG) greatly accelerated the reaction, which was best described by two initial equilibrium steps followed by one irreversible chemical step and a final product release equilibrium step. The irreversible step may correspond to β-elimination since it is the very step facilitated by 8-BrG.
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DOI: 10.1093/nar/gki694
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Nevinsky</name><affiliation wicri:level="1"><country xml:lang="fr">Russie</country><wicri:regionArea>Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences Novosibirsk 630090</wicri:regionArea><wicri:noRegion>Siberian Branch of the Russian Academy of Sciences Novosibirsk 630090</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Russie</country><wicri:regionArea>Novosibirsk State University Novosibirsk 630090</wicri:regionArea></affiliation></author><author><name sortKey="Douglas, Kenneth T" sort="Douglas, Kenneth T" uniqKey="Douglas K" first="Kenneth T." last="Douglas">Kenneth T. Douglas</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>School of Pharmacy and Pharmaceutical Sciences, University of Manchester Manchester M13 9PL</wicri:regionArea><wicri:noRegion>University of Manchester Manchester M13 9PL</wicri:noRegion></affiliation></author><author><name sortKey="Fedorova, Olga S" sort="Fedorova, Olga S" uniqKey="Fedorova O" first="Olga S." last="Fedorova">Olga S. 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Acids Res.</title><idno type="ISSN">0305-1048</idno><idno type="eISSN">1362-4962</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2005">2005</date><biblScope unit="volume">33</biblScope><biblScope unit="issue">12</biblScope><biblScope unit="page" from="3919">3919</biblScope><biblScope unit="page" to="3931">3931</biblScope></imprint><idno type="ISSN">0305-1048</idno></series><idno type="istex">6B642D7747AF57083C71DF5F25E174D0E5850A1D</idno><idno type="DOI">10.1093/nar/gki694</idno><idno type="local">gki694</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0305-1048</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Human 8-oxoguanine-DNA glycosylase (hOgg1) excises 8-oxo-7,8-dihydroguanine (8-oxoG) from damaged DNA. We report a pre-steady-state kinetic analysis of hOgg1 mechanism using stopped-flow and enzyme fluorescence monitoring. The kinetic scheme for hOgg1 processing an 8-oxoG:C-containing substrate was found to include at least three fast equilibrium steps followed by two slow, irreversible steps and another equilibrium step. The second irreversible step was rate-limiting overall. By comparing data from Ogg1 intrinsic fluorescence traces and from accumulation of products of different types, the irreversible steps were attributed to two main chemical steps of the Ogg1-catalyzed reaction: cleavage of the N-glycosidic bond of the damaged nucleotide and β-elimination of its 3′-phosphate. The fast equilibrium steps were attributed to enzyme conformational changes during the recognition of 8-oxoG, and the final equilibrium, to binding of the reaction product by the enzyme. hOgg1 interacted with a substrate containing an aldehydic AP site very slowly, but the addition of 8-bromoguanine (8-BrG) greatly accelerated the reaction, which was best described by two initial equilibrium steps followed by one irreversible chemical step and a final product release equilibrium step. The irreversible step may correspond to β-elimination since it is the very step facilitated by 8-BrG.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li><li>Russie</li></country></list><tree><country name="Russie"><noRegion><name sortKey="Kuznetsov, Nikita A" sort="Kuznetsov, Nikita A" uniqKey="Kuznetsov N" first="Nikita A." last="Kuznetsov">Nikita A. Kuznetsov</name></noRegion><name sortKey="Fedorova, Olga S" sort="Fedorova, Olga S" uniqKey="Fedorova O" first="Olga S." last="Fedorova">Olga S. Fedorova</name><name sortKey="Fedorova, Olga S" sort="Fedorova, Olga S" uniqKey="Fedorova O" first="Olga S." last="Fedorova">Olga S. Fedorova</name><name sortKey="Fedorova, Olga S" sort="Fedorova, Olga S" uniqKey="Fedorova O" first="Olga S." last="Fedorova">Olga S. Fedorova</name><name sortKey="Koval, Vladimir V" sort="Koval, Vladimir V" uniqKey="Koval V" first="Vladimir V." last="Koval">Vladimir V. Koval</name><name sortKey="Koval, Vladimir V" sort="Koval, Vladimir V" uniqKey="Koval V" first="Vladimir V." last="Koval">Vladimir V. Koval</name><name sortKey="Kuznetsov, Nikita A" sort="Kuznetsov, Nikita A" uniqKey="Kuznetsov N" first="Nikita A." last="Kuznetsov">Nikita A. Kuznetsov</name><name sortKey="Nevinsky, Georgy A" sort="Nevinsky, Georgy A" uniqKey="Nevinsky G" first="Georgy A." last="Nevinsky">Georgy A. Nevinsky</name><name sortKey="Nevinsky, Georgy A" sort="Nevinsky, Georgy A" uniqKey="Nevinsky G" first="Georgy A." last="Nevinsky">Georgy A. Nevinsky</name><name sortKey="Zharkov, Dmitry O" sort="Zharkov, Dmitry O" uniqKey="Zharkov D" first="Dmitry O." last="Zharkov">Dmitry O. Zharkov</name><name sortKey="Zharkov, Dmitry O" sort="Zharkov, Dmitry O" uniqKey="Zharkov D" first="Dmitry O." last="Zharkov">Dmitry O. Zharkov</name></country><country name="Royaume-Uni"><noRegion><name sortKey="Douglas, Kenneth T" sort="Douglas, Kenneth T" uniqKey="Douglas K" first="Kenneth T." last="Douglas">Kenneth T. Douglas</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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